NIFK antibody

Catalog No. ABIN498157

Product Details anti-NIFK Antibody

Target: NIFK (MKI67IP) (MKI67 FHA Domain-Interacting Nucleolar Phosphoprotein (MKI67IP))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NIFK antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of NIFK protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for NIFK

Target: NIFK (MKI67IP) (MKI67 FHA Domain-Interacting Nucleolar Phosphoprotein (MKI67IP))

Alternative Name: NIFK (MKI67IP Products)

Synonyms: NIFK antibody, Nopp34 antibody, AI852665 antibody, C130020J04Rik antibody, cb131 antibody, chunp6918 antibody, fb34b01 antibody, mki67ipl antibody, sb:cb131 antibody, wu:fb34b01 antibody, MKI67 FHA domain-interacting nucleolar phosphoprotein antibody, hypothetical protein antibody, nucleolar protein interacting with the FHA domain of MKI67 antibody, MKI67 (FHA domain) interacting nucleolar phosphoprotein antibody, EDI_059390 antibody, VDBG_05329 antibody, MGYG_01823 antibody, PGTG_07909 antibody, NIFK antibody, Nifk antibody, MKI67IP antibody, nifk antibody

Background: The structural proteins for the complex metalloenzyme nitrogenase include NIFK, NIFD and NIFH. These proteins are all necessary for archaeal and bacterial nitrogen fixation. The NIFK gene encodes the b subunit of the nitrogenase molybdenum-iron (MoFe) tetramer. NIFK localizes to the nucleolus where it interactes with the fork-head associated domain of the proliferation marker protein Ki-67 in a mitosis-specific and phosphorylation-dependent manner. NIFK is widely expressed in adult tissues, suggesting other functions in addition to its interaction with Ki-67, which is only expressed in proliferating cells.Synonyms: MKI67IP, NOPP34, Nucleolar phosphoprotein Nopp34, Nucleolar protein interacting with the FHA domain of pKI-67

Gene ID: 84365

NCBI Accession: NP_115766

UniProt: Q9BYG3