RYR2 antibody

Catalog No. ABIN498223

Product Details anti-RYR2 Antibody

Target: RYR2 (Ryanodine Receptor 2 (Cardiac) (RYR2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RYR2 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of RyR2 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Immunhistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RYR2

Target: RYR2 (Ryanodine Receptor 2 (Cardiac) (RYR2))

Alternative Name: Ryanodine Receptor 2 / RYR2 (RYR2 Products)

Synonyms: RYR2 antibody, ARVC2 antibody, ARVD2 antibody, RYR-2 antibody, RyR antibody, VTSIP antibody, 9330127I20Rik antibody, ryanodine receptor 2 antibody, ryanodine receptor 2, cardiac antibody, RYR2 antibody, Ryr2 antibody

Background: Dihydropyridine receptor (DHPR) is a surface membrane protein critical for the excitation-contraction coupling of striated muscle. DHPR and the sarcoplasmic reticulum ryanodine receptor (RyR) are two key components of the intracellular junctions, where depolarization of the surface membrane is converted into the release of Ca2+ from internal stores. The α1-subunit of the DHPR contains a cytoplasmic loop which is thought to be involved in the interactions with RyR. Phosphorylation of the DHPR α1-subunit is also thought to play a role in the functional interaction of DHPR and RyR. Mutation in DHPR α1 results in excitation-contraction uncoupling, leading to muscular dysgenesis, a complete inactivity in developing skeletal muscles. Cells that do not express RyR also lack excitation-contraction coupling and exhibit a severalfold reduction in Ca2+ current density.Synonyms: Cardiac muscle ryanodine receptor, Cardiac muscle ryanodine receptor-calcium release channel, Type 2 ryanodine receptor

Molecular Weight: approx. 565 kDa

Gene ID: 6262

NCBI Accession: NP_001026

UniProt: Q92736

Pathways: Myometrial Relaxation and Contraction