GTF2A1 antibody

Catalog No. ABIN498320

Product Details anti-GTF2A1 Antibody

Target: GTF2A1 (General Transcription Factor IIA, 1, 19/37kDa (GTF2A1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of TFIIA-α protein. (region surrounding Glu311)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for GTF2A1

Target: GTF2A1 (General Transcription Factor IIA, 1, 19/37kDa (GTF2A1))

Alternative Name: GTF2A1 / TF2A1 (GTF2A1 Products)

Synonyms: gtf2a1 antibody, tf2a1 antibody, tfiia antibody, tfiiaa/b antibody, tfiiaa/b-2 antibody, tf2a antibody, fc15h02 antibody, fj36b10 antibody, zgc:55847 antibody, wu:fc15h02 antibody, wu:fj36b10 antibody, GTF2A1 antibody, tfiiaa/b-1 antibody, 6330549H03Rik antibody, AA536742 antibody, AA959775 antibody, AW060250 antibody, TfIIAa/b antibody, Tfiia1 antibody, Tf2a antibody, TfIIa antibody, TF2A1 antibody, TFIIA antibody, TFIIA-42 antibody, TFIIAL antibody, general transcription factor IIA subunit 1 antibody, general transcription factor IIA 1 L homeolog antibody, general transcription factor IIA, 1 antibody, general transcription factor IIA 1 antibody, general transcription factor IIA 1 S homeolog antibody, general transcription factor II A, 1 antibody, general transcription factor 2A subunit 1 antibody, GTF2A1 antibody, gtf2a1.L antibody, gtf2a1 antibody, gtf2a1.S antibody, Gtf2a1 antibody

Background: Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP, one of the first steps in transcription initiation, may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-α, TFIIA-β and TFIIA-γ, and it interacts with both TBP and a TAF (TBP-associated factor). It has been demonstrated that the basic region of TBP is essential for TFIIA-dependent function of TBP.Synonyms: General transcription factor IIA subunit 1, TFIIA-42, TFIIAL, Transcription initiation factor IIA subunit 1

Molecular Weight: approx. 42 kDa

Gene ID: 2957

NCBI Accession: NP_056943

UniProt: P52655