IRX1 antibody
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- Target See all IRX1 Antibodies
- IRX1 (Iroquois Homeobox Protein 1 (IRX1))
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This IRX1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of IRX1 protein. (region surrounding Asp231)
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse.
Species reactivity (tested):Human. - Purification
- Affinity chromatography using epitope-specific immunogen
- Purity
- > 95 % (by SDS-PAGE)
- Top Product
- Discover our top product IRX1 Primary Antibody
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- Application Notes
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Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- Target
- IRX1 (Iroquois Homeobox Protein 1 (IRX1))
- Alternative Name
- IRX1 / IRXA1 (IRX1 Products)
- Synonyms
- IRX-5 antibody, IRXA1 antibody, IRX1 antibody, Xiro antibody, Xiro1 antibody, iro1 antibody, irx-5 antibody, irx1 antibody, zgc:109702 antibody, zgc:136490 antibody, ziro1 antibody, ziro1a antibody, Xiro-1 antibody, iro1-A antibody, iro1-B antibody, irx1-a antibody, irx1-b antibody, iroquois homeobox 1 antibody, Iroquois related homeobox 1 (Drosophila) antibody, iroquois homeobox 1a antibody, iroquois homeobox 1 L homeolog antibody, Putative iroquois-class homeodomain protein irx-1 antibody, iroquois homeobox 1 S homeolog antibody, IRX1 antibody, Irx1 antibody, irx1 antibody, irx1a antibody, irx1.L antibody, irx-1 antibody, irx1.S antibody
- Background
- The Iroquois homeobox gene family of transcription factors regulate aspects of embryonic development including anterior/posterior and dorsal/ventral axis patterning in the central nervous system. The Iroquois family are clustered on two loci, IRXA and IRXB, which map to chromosomes 8 and 13 in mice. The IRXA group includes Irx1, Irx2 and Irx4, the IRXB group is comprises Irx3, Irx5 and Irx6. Irx1 and Irx2 are both widely expressed during development in the lung epithelium and also in the ventricular septum. Irx1 and Irx2 also play a role in digit formation (E11.5-E14.5). The Irx gene family members are each expressed in a distinct pattern during mouse heart development. Specifically, Irx1 and Irx2 are expressed in the ventricular septum and Irx3 is expressed in the ventricular trabeculated myocardium. In addition, Irx4 is expressed in the linear heart tube and the AV canal, Irx5 is expressed in the endocardium lining the ventricular and atrial myocardium. Furthermore, the IRX4 gene may modulate cardiac development and function. Although the heart of Irx4- mice appears to develop normally, adult Irx4- mice exhibit cardiomyopathy, including cardiac hypertrophy and decreased contractility.Synonyms: Homeodomain protein IRXA1, IRX-1, Iroquois homeobox protein 1, Iroquois-class homeodomain protein IRX-1
- Molecular Weight
- approx. 47 kDa
- Gene ID
- 79192
- NCBI Accession
- NP_077313
- UniProt
- P78414
- Pathways
- Tube Formation
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