TCEB3 antibody

Catalog No. ABIN498446

Product Details anti-TCEB3 Antibody

Target: TCEB3 (Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, Elongin A) (TCEB3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCEB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Elongin A1 protein. (region surrounding Arg129)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TCEB3

Target: TCEB3 (Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, Elongin A) (TCEB3))

Alternative Name: TCEB3 (TCEB3 Products)

Synonyms: CG6755 antibody, Dmel\\CG6755 antibody, Elo-A antibody, dEloA antibody, tceb3a antibody, zgc:56638 antibody, wu:fw66g10 antibody, TCEB3 antibody, 110kDa antibody, AA408125 antibody, Tceb3a antibody, El antibody, EloA antibody, SIII antibody, SIII p110 antibody, TCEB3A antibody, Elongin A antibody, transcription elongation factor B polypeptide 3 antibody, elongin A antibody, calcium binding and coiled-coil domain 1b antibody, elongin A S homeolog antibody, transcription elongation factor B subunit 3 antibody, EloA antibody, LOC413407 antibody, eloa antibody, calcoco1b antibody, ELOA antibody, eloa.S antibody, Eloa antibody, TCEB3 antibody

Background: Individuals harboring germline mutations in the tumor suppressor gene von Hippel-Lindau (VHL) exhibit an increased susceptibility to a variety of tumors including renal carcinom,a hemangio-blastoma of the central nervous system and pheochromo-cytoma. The Elongin (SIII) complex has been identified as the functional target of the VHL protein. Elongin (SIII) is a heterotrimer composed of a transcriptional active subunit designated Elongin A and two regulatory subunits designated Elongin B and Elongin C. VHL functions by binding to the Elongin B and C subunits, inhibiting the transcriptional efficacy of the Elongin (SIII) complex. The VHL protein migrates with an apparent molecular weight of 38 kDa. The Elongin A subunit is 773 amino acids in length and has an apparent molecular weight of 110 kD,a while Elongin B and C are 18 kDa and 15 kDa proteins, respectively.Synonyms: EloA, Elongin 110 kDa subunit, Elongin A, Elongin-A, RNA polymerase II transcription factor SIII subunit A1, Transcription elongation factor B polypeptide 3

Molecular Weight: approx. 78 kDa

Gene ID: 6924

NCBI Accession: NP_003189

UniProt: Q14241