Stathmin 1 antibody (pSer25)

Catalog No. ABIN498736

Product Details anti-Stathmin 1 Antibody

Target: Stathmin 1 (STMN1)

Binding Specificity: pSer25

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Stathmin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Op18 protein only when phosphorylated at Ser25.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS, pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Stathmin 1

Target: Stathmin 1 (STMN1)

Alternative Name: Stathmin / STMN1 (STMN1 Products)

Synonyms: C1orf215 antibody, LAP18 antibody, Lag antibody, OP18 antibody, PP17 antibody, PP19 antibody, PR22 antibody, SMN antibody, 19k antibody, Lap18 antibody, Op18 antibody, P18 antibody, P19 antibody, Pig antibody, Pp17 antibody, Pp18 antibody, Pp19 antibody, Pr22 antibody, Smn antibody, prosolin antibody, op18 antibody, stathmin antibody, stmn1 antibody, stmn1a antibody, lag antibody, lap18 antibody, pp17 antibody, pp19 antibody, pr22 antibody, smn antibody, fj43c10 antibody, wu:fj38b07 antibody, wu:fj43c10 antibody, zgc:110159 antibody, cb959 antibody, wu:fb14e04 antibody, zgc:136942 antibody, stmn1-a antibody, stmn1b antibody, xo35 antibody, stathmin 1 antibody, stathmin 1 S homeolog antibody, stathmin 1b antibody, stathmin 1a antibody, stathmin-like antibody, stathmin antibody, stathmin 1 L homeolog antibody, STMN1 antibody, Stmn1 antibody, stmn1.S antibody, stmn1 antibody, stmn1b antibody, stmn1a antibody, LOC100229194 antibody, stmn1.L antibody

Background: Oncoprotein 18 (Op18) is a regulator of microtubule (MT) dynamics. Op18 is a target for both cell cycle and cell surface receptor-coupled kinase systems, and phosphorylation of Op18 on specific combinations of amino acid residues has been shown to switch off MT-destabilizing activity of Op18. Induced expression of the catalytic subunit of cAMPdependent protein kinase (PKA) results in a dramatic increase in cellular MT polymer content concomitant with phosphorylation and partial degradation of Op18. Phosphorylation of Op18 on two specific sites, Serine 16 and Serine 63, is necessary and sufficient for PKA to switch off Op18 activity in intact cells. PKA phosphorylation down-regulates the MT-destabilizing activity of Op18, which in turn promotes increased Tubulin polymerization. Op18 has the potential to regulate the MT system in response to external signals such as cAMP-linked agonists.Synonyms: LAP18, Leukemia-associated phosphoprotein p18, Metablastin, OP18, Oncoprotein 18, Op18, Phosphoprotein p19, Prosolin, Protein Pr22, pp17, pp19

Molecular Weight: approx. 19 kDa

Gene ID: 3925

NCBI Accession: NP_005554

UniProt: P16949

Pathways: MAPK Signaling, Microtubule Dynamics