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CPI-17 antibody (pThr38)

PPP1R14A Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN498756
  • Target See all CPI-17 (PPP1R14A) Antibodies
    CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))
    Binding Specificity
    • 15
    • 15
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pThr38
    Reactivity
    • 59
    • 24
    • 23
    • 5
    • 3
    • 3
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 55
    • 5
    Rabbit
    Clonality
    • 54
    • 6
    Polyclonal
    Conjugate
    • 30
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CPI-17 antibody is un-conjugated
    Application
    • 39
    • 21
    • 14
    • 14
    • 13
    • 12
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    This antibody detects endogenous levels of CPI-17 protein only when phosphorylated at Thr38.
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.
    Purification
    Affinity chromatography
    Purity
    > 95 % by SDS-PAGE
    Top Product
    Discover our top product PPP1R14A Primary Antibody
  • Application Notes
    Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1,0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))
    Alternative Name
    PPP1R14A (PPP1R14A Products)
    Synonyms
    MGC81874 antibody, cpi-17 antibody, ppp1inl antibody, CPI-17 antibody, im:7147597 antibody, ppp1r14a antibody, zgc:110700 antibody, PPP1R14A antibody, CPI17 antibody, PPP1INL antibody, Cpi17 antibody, 1110001M11Rik antibody, protein phosphatase 1 regulatory inhibitor subunit 14A L homeolog antibody, protein phosphatase 1 regulatory inhibitor subunit 14A antibody, protein phosphatase 1, regulatory (inhibitor) subunit 14Aa antibody, protein phosphatase 1, regulatory (inhibitor) subunit 14A antibody, ppp1r14a.L antibody, ppp1r14a antibody, ppp1r14aa antibody, PPP1R14A antibody, Ppp1r14a antibody
    Background
    CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle Myosin phosphate. CPI-17 was originally identified as a 17 kDa PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonin 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle cont-raction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase.Synonyms: 17 kDa PKC-potentiated inhibitory protein of PP1, CPI-17, CPI17, PPP1INL, Protein phosphatase 1 regulatory subunit 14A
    Molecular Weight
    approx. 22 kDa
    Gene ID
    94274
    NCBI Accession
    NP_150281
    UniProt
    Q96A00
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