N4BP1 antibody

Catalog No. ABIN498976

Product Details anti-N4BP1 Antibody

Target: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This N4BP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of N4BP1 protein. (region surrounding Tyr415)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for N4BP1

Target: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Alternative Name: N4BP1 (N4BP1 Products)

Synonyms: AI481586 antibody, C81621 antibody, RGD1305179 antibody, wu:fi03b05 antibody, zgc:66437 antibody, NEDD4 binding protein 1 antibody, Nedd4 binding protein 1 antibody, nedd4 binding protein 1 antibody, nedd4 binding protein 1 L homeolog antibody, N4BP1 antibody, N4bp1 antibody, n4bp1 antibody, n4bp1.L antibody

Background: Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin-protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73alpha, a target of Itchmediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73alpha for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73alpha and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.Synonyms: KIAA0615, NEDD4-binding protein 1

Molecular Weight: approx. 100 kDa

Gene ID: 9683

NCBI Accession: NP_694574

UniProt: O75113