STAT4 antibody (Signal Transducer and Activator of Transcription 4) (Internal Region)

Details for Product anti-STAT4 Antibody No. ABIN499124, Supplier: Log in to see
Antigen
  • SLEB11
  • signal transducer and activator of transcription 4
  • STAT4
  • stat4
  • Stat4
Alternatives
anti-Human STAT4 antibody for Enzyme Immunoassay
Epitope
Internal Region, AA 613-627
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Reactivity
Dog (Canine), Human, Pig (Porcine)
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Host
Goat
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Clonality
Polyclonal
Conjugate
This STAT4 antibody is un-conjugated
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Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen Peptide with sequence from the internal region (aa 613-627) of the protein sequence according to Human STAT4 (NP_003142.1.) Genename: STAT4
Sequence C-DHSESGEVRF HSVEP
Specificity Recognizes STAT4 (aa 613-627).
Cross-Reactivity (Details) Species reactivity (expected):Canine, Pig.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Plasmids, Primers & others Plasmids, Primers & others STAT4 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name STAT4 (STAT4 Antibody Abstract)
Background The STAT 4 protein is a transcription factor activated by hormone and cytokines. STAT 4 is most similar to STAT 1 (52 % ) and STAT 3 (47 %). Functionally STAT 4 is similar to other STAT family members in that it can be tyrosine phosphorylated by JAK 1 or JAK 2. STAT 4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT 4 can bind the interferon g activated site (GAS), and serine phosphorylation is also required for maximal transcriptional activity. STAT 4 expression is restricted to the thymus, spleen, and testis.Synonyms: Signal transducer and activator of transcription 4
Gene ID 6775
NCBI Accession NP_003142
UniProt Q14765
Pathways JAK-STAT Signaling
Application Notes Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.2-0.5 μg/mL. This antibody detects a band of approx. 85 kDa in transfectedHEK293 transiently expressing full-length Human STAT4 (MYC and DYKDDDDK tagged). No bands were observed in mock-transfected HEK293 and the same band was observedusing anti-myc tag antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 4 (STAT4) (AA 613-627), (Internal Region) antibody (ABIN499124) HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human STAT4 with C-termin...
Background publications Good, Thieu, Mathur, Yu, Stritesky, Yeh, OMalley, Perumal, Kaplan: "Temporal induction pattern of STAT4 target genes defines potential for Th1 lineage-specific programming." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 6, pp. 3839-47, 2009 (PubMed).

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