Shootin-1 (SHTN1) (AA 21-130) antibody (Alexa Fluor 680)

Catalog No. ABIN5010511

Product Details

Target: Shootin-1 (SHTN1)

Binding Specificity: AA 21-130

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Alexa Fluor 680

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Shootin/KIAA1598

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Shootin-1 (SHTN1)

Alternative Name: Shootin/KIAA1598 (SHTN1 Products)

Synonyms: Kiaa1598 antibody, Shootin1 antibody, mKIAA1598 antibody, shootin 1 antibody, SHTN1 antibody, Shtn1 antibody

Background:

Synonyms: DKFZp686A0439, hypothetical protein FLJ11122, Kiaa1598, MGC40476, Putative uncharacterized protein KIAA1598, Shootin 1, Shootin-1, SHOT1_HUMAN.

Background: Shootin1 is a 631 amino acid protein that belongs to the shootin family. The shootin1 protein contains three coiled-coil domains, a proline-rich region and interacts with RUFY3. Shootin1 is involved in the generation of internal asymmetric signals required for neuronal polarization. The shootin1 protein acts upstream of PI3K (phosphoinositide 3-kinase), by being required for spatially localized PI3K activity. By accumulating asymmetrically in a single neurite before polarization, shootin1 leads to axon induction for polarization, additionally the absence of shootin1 from the nascent axon's siblings by competition prevents the formation of surplus axons. Existing as seven alternatively spliced isoforms, the shootin1 gene is conserved in chimpanzee, dog, mouse, rat, chicken and zebrafish, and maps to human chromosome 10q25.3.