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Amyloid beta Precursor Protein Binding Protein 1 (AA 229-534) antibody

Reactivity: Human ICC, IHC, IP, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5013120
  • Target
    Amyloid beta Precursor Protein Binding Protein 1
    Binding Specificity
    AA 229-534
    Reactivity
    Human
    Host
    • 1
    Rabbit
    Clonality
    • 1
    Polyclonal
    Conjugate
    • 1
    Un-conjugated
    Application
    Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
    Purpose
    Polyclonal Antibody to Amyloid Beta Precursor Protein Binding Protein 1 (APPBP1)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against APPBP1. It has been selected for its ability to recognize APPBP1 in immunohistochemical staining and western blotting.
    Cross-Reactivity
    Mouse, Pig, Rat
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Amyloid Beta Precursor Protein Binding Protein 1 (APPBP1) corresdonding to Thr229~Leu534 with N-terminal His Tag
    Isotype
    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    1:160-640 Immunohistochemistry: 5-20 μg/mL

    1:16-64 Immunocytochemistry: 5-20 μg/mL

    1:16-64 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Naik, Lam, Parija, Prakash, Jiramongkol, Adhya, Parida, Mishra: "NEDDylation negatively regulates ERRβ expression to promote breast cancer tumorigenesis and progression." in: Cell death & disease, Vol. 11, Issue 8, pp. 703, (2020) (PubMed).

  • Target
    Amyloid beta Precursor Protein Binding Protein 1
    Synonyms
    A-116A10.1 antibody, APPBP1 antibody, ula-1 antibody, NEDD8 activating enzyme E1 subunit 1 antibody, NAE1 antibody
    Background
    NAE1, HPP1, Ula-1, NEDD8-Activating Enzyme E1 Regulatory Subunit 1, Amyloid protein-binding protein 1, Proto-oncogene protein 1
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