C9 antibody (AA 136-512)

Catalog No. ABIN5013382

Product Details anti-C9 Antibody

Target: C9 (Complement Component C9 (C9))

Binding Specificity: AA 136-512

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Complement Component 9 (C9)

Specificity: The antibody is a rabbit polyclonal antibody raised against C9. It has been selected for its ability to recognize C9 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Complement Component 9 (C9) corresdonding to Pro136~Arg512 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-C9 antibody (ABIN5013382)

Sones, Merriam, Seffens, Brown-Grant, Butler, Zhao, Xu, Shawber, Grenier, Douglas: "Angiogenic factor imbalance precedes complement deposition in placentae of the BPH/5 model of preeclampsia." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 32, Issue 5, pp. 2574-2586, (2018) (PubMed).

Fu, Ju, Lin, Xiao, Li, Zhuang, Zhang, Ma, Li, Ma, Su, Wang, Qin, Liang: "Target deletion of complement component 9 attenuates antibody-mediated hemolysis and lipopolysaccharide (LPS)-induced acute shock in mice." in: Scientific reports, Vol. 6, pp. 30239, (2016) (PubMed).

Target Details for C9

Target: C9 (Complement Component C9 (C9))

Alternative Name: Complement Component 9 (C9 Products)

Synonyms: LOC100037951 antibody, LOC100136130 antibody, c9 antibody, wu:fb60b05 antibody, wu:fd50e04 antibody, zgc:112272 antibody, complement C9 antibody, complement component 9 antibody, complement component 9 L homeolog antibody, C9 antibody, c9 antibody, c9.L antibody

Pathways: Complement System