POLD1 antibody (AA 41-254)

Catalog No. ABIN5014137

Product Details anti-POLD1 Antibody

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Binding Specificity: AA 41-254

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Polymerase DNA Directed Delta 1 (POLd)

Specificity: The antibody is a rabbit polyclonal antibody raised against POLd. It has been selected for its ability to recognize POLd in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Polymerase DNA Directed Delta 1 (POLd) corresdonding to Met41~Phe254 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:330-1300 Immunohistochemistry: 5-20 μg/mL

1:33-130 Immunocytochemistry: 5-20 μg/mL

1:33-130 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for POLD1

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Alternative Name: Polymerase DNA Directed Delta 1 (POLD1 Products)

Synonyms: CDC2 antibody, CRCS10 antibody, POLD antibody, 5949 antibody, CAA61369 antibody, CG5949 antibody, DNA Pol Delta antibody, Dm Poldelta antibody, Dmel\\CG5949 antibody, Poldelta antibody, dnapol delta antibody, l(3)72Ac antibody, cdc2 antibody, pold antibody, 125kDa antibody, Pol delta antibody, fla antibody, im:7139126 antibody, zgc:152688 antibody, DNA polymerase delta 1, catalytic subunit antibody, DNA-polymerase-delta antibody, polymerase (DNA directed), delta 1, catalytic subunit antibody, DNA polymerase delta catalytic subunit antibody, DNA polymerase delta antibody, POLD1 antibody, DNApol-delta antibody, pold1 antibody, LOC100456571 antibody, Pold1 antibody

Background: CDC2, POLD1, DNA polymerase subunit delta p125

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Chromatin Binding, Synthesis of DNA