SEMA4D/CD100 antibody (AA 145-494)

Catalog No. ABIN5014300

Product Details anti-SEMA4D/CD100 Antibody

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Binding Specificity: AA 145-494

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEMA4D/CD100 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Semaphorin 4D (SEMA4D)

Specificity: The antibody is a rabbit polyclonal antibody raised against SEMA4D. It has been selected for its ability to recognize SEMA4D in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Semaphorin 4D (SEMA4D) corresdonding to Leu145~Gly494 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:150-600 Immunohistochemistry: 5-20 μg/mL

1:15-60 Immunocytochemistry: 5-20 μg/mL

1:15-60 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SEMA4D/CD100

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Alternative Name: Semaphorin 4D (SEMA4D Products)

Synonyms: C9orf164 antibody, CD100 antibody, M-sema-G antibody, SEMAJ antibody, coll-4 antibody, Semacl2 antibody, Semaj antibody, Semcl2 antibody, COLL-4 antibody, semaphorin 4D antibody, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D antibody, Sema4d antibody, SEMA4D antibody

Background: CD100, A8, BB18, GR3, M-Sema G, M-Sema-G, SEMAJ, Coll-4, Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D

Pathways: Regulation of Cell Size