Vangl1 antibody (AA 148-400)

Catalog No. ABIN5027627

Product Details anti-Vangl1 Antibody

Target: Vangl1 (Vang-Like 1 (Vangl1))

Binding Specificity: AA 148-400

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vangl1 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Protein A Chromatography

Immunogen: A partial length recombinant Vang like-1 protein (amino acids 148-400) was used as the immunogen for this antibody.

Isotype: IgG

Application Details

Application Notes: WB: 2-4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for Vangl1

Target: Vangl1 (Vang-Like 1 (Vangl1))

Alternative Name: Vang like-1 (Vangl1 Products)

Background: Vangl1 (VANGL planar cell polarity protein 1) is an integral membrane protein that belongs to the tetraspanin family having four transmembrane domains. Tetraspanins form large multimolecular complexes on the cell membrane and are involved in cellular signaling, membrane formation, and migration. Vangl1 is expressed at the plasma membrane of different tissues and play a critical role in establishing planar cell polarity (PCP) in epithelial cells and are required for convergent extension (CE) movements during embryogenesis. It is a highly conserved and have evolved from a single ancestral protein Strabismus/Van Gogh found in Drosophila. Mutations in the vangl1 gene cause NTDs (neural tube defects). Ser/Thr phosphorylation of Vangl1 serves as an effector mediating the ITF healing response of the intestinal mucosa. In colon carcinoma cell lines, Vangl1 interacts with KAI1 (CD82) tumor metastasis suppressor. Vangl1 overexpression increases motility and metastasis, whereas its inhibition decreases motility and metastasis of colon cancer confirming migratory phenotype for Vangl1 in colonic epithelial cells.

Molecular Weight: 62 kDa

Gene ID: 81839

UniProt: Q8TAA9

Pathways: WNT Signaling