ATG7 antibody (N-Term) (Alkaline Phosphatase (AP))

Catalog No. ABIN5065894

Product Details anti-ATG7 Antibody (Alkaline Phosphatase (AP))

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG7 antibody is conjugated to Alkaline Phosphatase (AP)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.,Detects 77.9 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide from the N-terminal of Human ATG7

Isotype: IgG

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5065894 was sufficient for detection of ATG7 in 15 μg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: ATG7 (ATG7 Products)

Synonyms: APG7-LIKE antibody, APG7L antibody, GSA7 antibody, D8 antibody, UBA1B antibody, UBE1L antibody, UBE2 antibody, Ube1l antibody, 1300004C08Rik antibody, AO090001000744 antibody, atg7 antibody, 1810013K23Rik antibody, Agp7 antibody, Apg7l antibody, Atg7l antibody, ATAPG7 antibody, ATATG7 antibody, ATG7 antibody, AUTOPHAGY 7 antibody, AUTOPHAGY-RELATED 7 antibody, K15I22.10 antibody, K15I22_10 antibody, autophagy related 7 antibody, ubiquitin like modifier activating enzyme 7 antibody, ubiquitin-like modifier activating enzyme 7 antibody, autophagy-related protein 7 antibody, Autophagy-related protein 7 antibody, Apg7p antibody, ThiF family protein antibody, ubiquitin-like conjugating enzyme Atg7 antibody, ATG7 antibody, UBA7 antibody, Uba7 antibody, ANI_1_3368024 antibody, AOR_1_1338164 antibody, PTRG_02236 antibody, PAAG_00287 antibody, PITG_12537 antibody, atg7 antibody, Atg7 antibody, APG7 antibody

Background: ATG7 in conjuction with ATG10, mediates the formation of the autophagosome when ATG12 is covalently bound to ATG5 and targets to autophagosome vesicles. It also activates ATG8, and is crucial for amino acid supply in neonates.

Gene ID: 10533

NCBI Accession: NP_001129503

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy