SQSTM1 antibody (AA 335-345) (PE)
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- Target See all SQSTM1 Antibodies
- SQSTM1 (Sequestosome 1 (SQSTM1))
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Binding Specificity
- AA 335-345
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SQSTM1 antibody is conjugated to PE
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Application
- Western Blotting (WB)
- Specificity
- Ubiquitously expressed.,Detects ~60 kDa. Band at 40 kDa is isoform 2.
- Cross-Reactivity
- Human, Mouse
- Purification
- Peptide Affinity Purified
- Immunogen
- Synthetic peptide from the C-terminal of Human SQSTM1 (aa. 335-345)
- Isotype
- IgG
- Top Product
- Discover our top product SQSTM1 Primary Antibody
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- Application Notes
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- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
- Comment
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A 1:1000 dilution of ABIN5066117 was sufficient for detection of SQSTM1 in 15 μg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- SQSTM1 (Sequestosome 1 (SQSTM1))
- Alternative Name
- SQSTM1 (SQSTM1 Products)
- Synonyms
- SQSTM1 antibody, sqstm1 antibody, MGC79491 antibody, A170 antibody, OSIL antibody, PDB3 antibody, ZIP3 antibody, p60 antibody, p62 antibody, p62B antibody, OSF-6 antibody, Osi antibody, STAP antibody, ZIP antibody, sb:cb621 antibody, zgc:85784 antibody, sequestosome 1 antibody, sequestosome 1 L homeolog antibody, SQSTM1 antibody, sqstm1 antibody, Sqstm1 antibody, sqstm1.L antibody
- Background
- SQSTM1 or Sequestome 1 (p62) is a ubiquitin binding protein that interacts with ubiqutin, providing a scaffold for several signaling proteins and triggering degradation of proteins throught the proteasome or lysossome. This gene regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Mutations in this gene result in sporadic and familial Paget disease of bone. SQSTM1 also binds autophagosomal membrane protein LC3/ATG8.
- Gene ID
- 8878
- NCBI Accession
- NP_001135770
- UniProt
- Q13501
- Pathways
- NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy
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