GDNF antibody (Atto 488)

Catalog No. ABIN5066751

Product Details anti-GDNF Antibody (Atto 488)

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GDNF antibody is conjugated to Atto 488

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.,Detects ~24 kDa.

Cross-Reactivity: Human, Rat

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide from the mid-protein of Human GDNF

Isotype: IgG

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5066751 was sufficient for detection of GDNF in 15 μg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for GDNF

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Alternative Name: GDNF (GDNF Products)

Synonyms: gdnf antibody, GDNF antibody, AI385739 antibody, gdnfa antibody, hfb1-gdnf antibody, ATF1 antibody, ATF2 antibody, HFB1-GDNF antibody, HSCR3 antibody, glial cell derived neurotrophic factor antibody, glial cell derived neurotrophic factor a antibody, glial cell line derived neurotrophic factor antibody, glial cell derived neurotrophic factor L homeolog antibody, Gdnf antibody, gdnfa antibody, GDNF antibody, gdnf.L antibody

Gene ID: 2668

NCBI Accession: NP_000505

UniProt: P39905

Pathways: RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration