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HSP70 antibody (Atto 390)

HSP70 Reactivity: Human WB, IF, ICC, FACS Host: Mouse Monoclonal 1H11 Atto 390
Catalog No. ABIN5066930
  • Target See all HSP70 Antibodies
    HSP70 (Heat Shock Protein 70 (HSP70))
    Reactivity
    • 319
    • 197
    • 163
    • 71
    • 60
    • 53
    • 52
    • 52
    • 36
    • 31
    • 29
    • 29
    • 28
    • 23
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    • 15
    • 13
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    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 206
    • 146
    • 13
    • 11
    • 11
    Mouse
    Clonality
    • 228
    • 158
    Monoclonal
    Conjugate
    • 109
    • 31
    • 28
    • 26
    • 24
    • 20
    • 19
    • 18
    • 18
    • 18
    • 12
    • 5
    • 5
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    • 5
    • 5
    • 4
    • 4
    • 4
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    • 3
    • 2
    • 1
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    • 1
    This HSP70 antibody is conjugated to Atto 390
    Application
    • 371
    • 159
    • 140
    • 122
    • 120
    • 109
    • 98
    • 66
    • 62
    • 61
    • 41
    • 33
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    • 6
    • 4
    • 2
    • 2
    • 1
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    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Specificity
    Induced by heat shock.,Conformation-spec ab for mem-bound HSP70. Binds to cell surface HSP70 w/o permeabilization of cell mem in tumor cells. Does't bind to Intra-C HSP70 at low conc. X-reacts with HSC70. ~70 kDa in WB.
    Cross-Reactivity
    Cow, Human, Mouse, Pig, Rat
    Purification
    Protein G Purified
    Immunogen
    Human native HSP70 protein
    Clone
    1H11
    Isotype
    IgG1
    Top Product
    Discover our top product HSP70 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:100)
    • FACS (1:250)
    • optimal dilutions for assays should be determined by the user.
    Comment

    A 1:250 dilution of ABIN5066930 was sufficient for detection of cell surface HSP70 in HCT116 cells using Fluorescence-activated cell sorting, applied in flow cytometry, with FITC as the fluorescent probe.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    HSP70 (Heat Shock Protein 70 (HSP70))
    Alternative Name
    HSP70 (HSP70 Products)
    Synonyms
    APG-2 antibody, HS24/P52 antibody, HSPH2 antibody, RY antibody, hsp70 antibody, hsp70RY antibody, CG31354 antibody, HSP70 antibody, Hsp70Bb antibody, hsp70B antibody, hsp70Bb-prime antibody, DmelCG5834 antibody, CG5834 antibody, HSPA1 antibody, HSP70B' antibody, HSPA6 antibody, ARABIDOPSIS HEAT SHOCK PROTEIN 70 antibody, ATHSP70 antibody, heat shock protein 70 antibody, LOC100305036 antibody, hsc70 antibody, Hsp70 antibody, Hsp70-1 antibody, Hsp70.1 antibody, hsp68 antibody, Hsp110 antibody, irp94 antibody, HSP70-2 antibody, HSPA1B antibody, HSPA2 antibody, hsp70-5 antibody, HSP70-1 antibody, HSP70.1 antibody, HSP70.2 antibody, heat shock protein family A (Hsp70) member 4 antibody, CG5834 gene product from transcript CG5834-RA antibody, heat shock protein 70 antibody, heat shock protein family A (Hsp70) member 6 antibody, heat shock 70kDa protein 2 antibody, heat shock 70 kD protein cognate antibody, Hsp70 family chaperone antibody, Heat shock protein 70 antibody, Heat shock protein 70, putative antibody, heat shock protein 1B antibody, heat shock protein family A member 4 antibody, heat shock 70kDa protein 1A antibody, heat shock protein 1 antibody, Heat Shock Protein antibody, heat shock cognate 70-kd protein antibody, Heat shock 70 kDa protein 1A antibody, HSPA4 antibody, Hsp70Bbb antibody, HSP70 antibody, HSPA6 antibody, HSPA2 antibody, PCC7424_2419 antibody, Isop_1041 antibody, CGB_C3390W antibody, Bacsa_1698 antibody, dnaK-B antibody, LOC100305036 antibody, Hspa1b antibody, Hspa4 antibody, HSPA1A antibody, hsp1 antibody, hsp-70 antibody, hsp70 antibody, LOC108348108 antibody
    Background
    HSP70 is a highly conserved protein that is ubiquitously expressed. It can be found in chloroplasts, eukaryotic cytosol, endoplasmic reticulum, and mitochondria, but also embedded in the cell membrane and in the extracellular space (1,2,3,4). Even though HSP70 is one of the most studied heat shock proteins, the export mechanism and method of membrane insertion are not fully understood. The majority of the proteins in the heat shock family lack a consensus signal for secretion via the ER-Golgi pathway (5). Researchers have found that HSP70 may be released from cells via exosomes or secretory vesicles (6). Although HSP70 is ubiquitously expressed, there is not much information about its presence on cell surface. The finding that HSP70 is localized on the cell surface of cancer cells but not normal cells suggests a conformational change of HSP70 in the lower pH environment characteristic of cancer cells (7). The presence of cell membrane embedded HSP70 has been found to increase the stability of cancer cells, thereby protecting tumors from environmental stress (8, 9). Anti-HSP70 antibody, clone 1H11 (Catalog No. SMC-249) is unique from other commercially available antibodies in that it can bind to the extracellular region of the cell membrane embedded HSP70 protein, allowing researchers to differentiate between membrane bound and intracellular HSP70 across cancer cells types.
    Gene ID
    3303
    NCBI Accession
    NP_005336
    UniProt
    P0DMV8, P0DMV9
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