DNMT1 antibody (AA 637-650)

Catalog No. ABIN531980

Product Details anti-DNMT1 Antibody

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Binding Specificity: AA 637-650

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Free Floating) (IHC (ff))

Purpose: Mouse monoclonal antibody raised against synthetic peptide of DNMT1.

Sequence: EKDDREDKEN AFKR

Cross-Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Immunogen: A synthetic peptide corresponding to amino acids 637-650 of human DNMT1.

Clone: 60B1220-1

Isotype: IgG1

Application Details

Application Notes: ChIP (1:20-1:1000)
Flow Cytometry
Immunocytochemistry (1:10)
Immunofluorescence (1:10)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:500)
Immunohistochemistry (Free floating sections) (1:500)
Immunohistoche

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.05 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-DNMT1 antibody (ABIN531980)

Du, Li, You, Wang, Wang, Cao, Hu, Liu: "DNA methylation in cystathionine-γ-lyase (CSE) gene promoter induced by ox-LDL in macrophages and in apoE knockout mice." in: Biochemical and biophysical research communications, Vol. 469, Issue 3, pp. 776-82, (2016) (PubMed).

Li, Li, Du, Wang, You, Wang, Xu, Cheng, Cao, Liu, Hu: "Homocysteine Triggers Inflammatory Responses in Macrophages through Inhibiting CSE-H2S Signaling via DNA Hypermethylation of CSE Promoter." in: International journal of molecular sciences, Vol. 16, Issue 6, pp. 12560-77, (2015) (PubMed).

Siouda, Frecha, Accardi, Yue, Cuenin, Gruffat, Manet, Herceg, Sylla, Tommasino: "Epstein-Barr virus down-regulates tumor suppressor DOK1 expression." in: PLoS pathogens, Vol. 10, Issue 5, pp. e1004125, (2014) (PubMed).

Saidj, Cros, Hernandez-Vargas, Guarino, Sylla, Tommasino, Accardi: "Oncoprotein E7 from beta human papillomavirus 38 induces formation of an inhibitory complex for a subset of p53-regulated promoters." in: Journal of virology, Vol. 87, Issue 22, pp. 12139-50, (2013) (PubMed).

Du, Zhou, Liu, Jiang, Wang, Hu, Qi, Zhong, Wang, Li: "Arsenic induces functional re-expression of estrogen receptor ? by demethylation of DNA in estrogen receptor-negative human breast cancer." in: PLoS ONE, Vol. 7, Issue 4, pp. e35957, (2012) (PubMed).

Cheng, Auersperg, Leung: "Inhibition of p53 represses E-cadherin expression by increasing DNA methyltransferase-1 and promoter methylation in serous borderline ovarian tumor cells." in: Oncogene, Vol. 30, Issue 37, pp. 3930-42, (2011) (PubMed).

Ji, Yang, Yu, Yuan, Hu, Zhang, Yang, Pang, Li, Lu, Fu, Chen, Lin, Chen, Zhuang: "Epigenetic silencing of O6-methylguanine DNA methyltransferase gene in NiS-transformed cells." in: Carcinogenesis, Vol. 29, Issue 6, pp. 1267-75, (2008) (PubMed).

Guastafierro, Cecchinelli, Zampieri, Reale, Riggio, Sthandier, Zupi, Calabrese, Caiafa: "CCCTC-binding factor activates PARP-1 affecting DNA methylation machinery." in: The Journal of biological chemistry, Vol. 283, Issue 32, pp. 21873-80, (2008) (PubMed).

Xia, Schneider-Stock, Diestel, Habold, Krueger, Roessner, Naumann, Lendeckel: "Helicobacter pylori regulates p21(WAF1) by histone H4 acetylation." in: Biochemical and biophysical research communications, Vol. 369, Issue 2, pp. 526-31, (2008) (PubMed).

Villa, Pasini, Gutierrez, Morey, Occhionorelli, Viré, Nomdedeu, Jenuwein, Pelicci, Minucci, Fuks, Helin, Di Croce: "Role of the polycomb repressive complex 2 in acute promyelocytic leukemia." in: Cancer cell, Vol. 11, Issue 6, pp. 513-25, (2007) (PubMed).

Mabaera, Greene, Richardson, Conine, Kozul, Lowrey: "Neither DNA hypomethylation nor changes in the kinetics of erythroid differentiation explain 5-azacytidine's ability to induce human fetal hemoglobin." in: Blood, Vol. 111, Issue 1, pp. 411-20, (2007) (PubMed).

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT1 (DNMT1 Products)

Synonyms: cb1075 antibody, cb983 antibody, dmnt1 antibody, fb05h01 antibody, mta antibody, wu:fb05h01 antibody, ADCADN antibody, AIM antibody, CXXC9 antibody, DNMT antibody, HSN1E antibody, MCMT antibody, DDM2 antibody, DECREASED DNA METHYLATION 2 antibody, DMT01 antibody, DMT1 antibody, DNA METHYLTRANSFERASE antibody, DNA METHYLTRANSFERASE 01 antibody, DNA METHYLTRANSFERASE 1 antibody, MET2 antibody, METHYLTRANSFERASE 2 antibody, METHYLTRANSFERASE I antibody, METI antibody, methyltransferase 1 antibody, Cxxc9 antibody, Dnmt antibody, Dnmt1o antibody, MTase antibody, Met-1 antibody, Met1 antibody, MommeD2 antibody, m.MmuI antibody, DNA methyltransferase 1 antibody, DNA (cytosine-5-)-methyltransferase 1 antibody, methyltransferase 1 antibody, DNA methyltransferase (cytosine-5) 1 antibody, DNMT1 antibody, dnmt1 antibody, Dnmt1 antibody, MET1 antibody

Gene ID: 1786

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection