Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Purpose
Mouse monoclonal antibody raised against partial recombinant NEDD9.
Specificity
This Protein A purified antibody is directed against human HEF1 protein. Reactivity against multiple isoforms is expected. Specificity was determined by partial epitope mapping.
Cross-Reactivity
Human
Characteristics
Antibody Reactive Against Recombinant Protein.
Purification
Purified
Immunogen
Recombinant GST fusion protein corresponding to amino acids 82-398 of human NEDD9.
NEDD9
Reactivity: Human
ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western Blot (1:500) Immunofluorescence (1:100) Immunoprecipitation (1:100) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Merrill, See, Wertheim, Clagett-Dame: "Crk-associated substrate (Cas) family member, NEDD9, is regulated in human neuroblastoma cells and in the embryonic hindbrain by all-trans retinoic acid." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 231, Issue 3, pp. 564-75, (2004) (PubMed).
Zheng, McKeown-Longo: "Regulation of HEF1 expression and phosphorylation by TGF-beta 1 and cell adhesion." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39599-608, (2002) (PubMed).