EPAS1 antibody (AA 535-631)

Catalog No. ABIN534071

Product Details anti-EPAS1 Antibody

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Binding Specificity: AA 535-631

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Mouse monoclonal antibody raised against partial recombinant EPAS1.

Specificity: This antibody is specific to HIF-2 alpha and does not cross-react with HIF-1 alpha.

Cross-Reactivity: Human, Mouse, Rat

Immunogen: Recombinant protein corresponding to amino acids 535-631 of human EPAS1.

Clone: Ep190b

Isotype: IgG1

Application Details

Application Notes: ELISA (1:1000)
Flow Cytometry (1 μg/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:150-1:300)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-EPAS1 antibody (ABIN534071)

Chintalapudi, Markiewicz, Kose, Dammai, Champion, Hoda, Trojanowska, Hsu: "Cyr61/CCN1 and CTGF/CCN2 mediate the proangiogenic activity of VHL-mutant renal carcinoma cells." in: Carcinogenesis, Vol. 29, Issue 4, pp. 696-703, (2008) (PubMed).

Kubo, Sugita, Shimose, Matsuo, Arihiro, Ochi: "Expression of hypoxia-inducible factor-1alpha and its relationship to tumour angiogenesis and cell proliferation in cartilage tumours." in: The Journal of bone and joint surgery. British volume, Vol. 90, Issue 3, pp. 364-70, (2008) (PubMed).

Bernhardt, Wiesener, Weidemann, Schmitt, Weichert, Lechler, Campean, Ong, Willam, Gretz, Eckardt: "Involvement of hypoxia-inducible transcription factors in polycystic kidney disease." in: The American journal of pathology, Vol. 170, Issue 3, pp. 830-42, (2007) (PubMed).

Sutphin, Chan, Li, Turcotte, Krieg, Giaccia: "Targeting the loss of the von Hippel-Lindau tumor suppressor gene in renal cell carcinoma cells." in: Cancer research, Vol. 67, Issue 12, pp. 5896-905, (2007) (PubMed).

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: HIF2A / HIF2 alpha (EPAS1 Products)

Synonyms: EPAS1 antibody, ECYT4 antibody, HIF2A antibody, HLF antibody, MOP2 antibody, PASD2 antibody, bHLHe73 antibody, HIF-2alpha antibody, HRF antibody, Hif2a antibody, bhlhe73 antibody, ecyt4 antibody, hif2 antibody, hif2a antibody, hif2alpha antibody, hlf antibody, mop2 antibody, pasd2 antibody, EPAS antibody, EPAS-1 antibody, endothelial PAS domain protein 1 antibody, endothelial PAS domain protein 1 L homeolog antibody, EPAS1 antibody, epas1 antibody, Epas1 antibody, epas1.L antibody

Gene ID: 2034

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect