NEDD9 antibody (AA 82-398)

Catalog No. ABIN534196

Product Details anti-NEDD9 Antibody

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Binding Specificity: AA 82-398

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEDD9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purpose: Mouse monoclonal antibody raised against partial recombinant NEDD9.

Specificity: Human, mouse, rat NEDD9. Does not recognize p130Cas. Have not tested on Sin1. This mAb mostly detects HEF1 localized at the focal adhesion sites.

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Antibody Reactive Against Recombinant Protein.

Purification: Purified

Immunogen: Recombinant GST fusion protein corresponding to amino acids 82-398 of human NEDD9.

Clone: 2G9

Isotype: IgG1

Application Details

Application Notes: Western Blot (1:1000)
Immunoprecipitation (1:2000-1:6000)
Immunofluorescence (1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-NEDD9 antibody (ABIN534196)

Liu, Wang, Zhao, Zhu, Yin, Wei, Wu, Cheng, Wang, Ni, Wang, Cao, Huang, Cai: "NEDD9 overexpression correlates with poor prognosis in gastric cancer." in: Tumour biology, Vol. 35, Issue 7, pp. 6351-6, (2014) (PubMed).

Regelmann, Danzl, Wanjalla, Alexandropoulos: "The hematopoietic isoform of Cas-Hef1-associated signal transducer regulates chemokine-induced inside-out signaling and T cell trafficking." in: Immunity, Vol. 25, Issue 6, pp. 907-18, (2006) (PubMed).

Bargon, Gunning, ONeill: "The Cas family docking protein, HEF1, promotes the formation of neurite-like membrane extensions." in: Biochimica et biophysica acta, Vol. 1746, Issue 2, pp. 143-54, (2005) (PubMed).

ONeill, Golemis: "Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics." in: Molecular and cellular biology, Vol. 21, Issue 15, pp. 5094-108, (2001) (PubMed).

Target Details for NEDD9

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Alternative Name: NEDD9 (NEDD9 Products)

Synonyms: NEDD9 antibody, CAS-L antibody, CAS2 antibody, CASL antibody, CASS2 antibody, HEF1 antibody, Cas-L antibody, CasL antibody, E230025G09Rik antibody, neural precursor cell expressed, developmentally down-regulated 9 antibody, neural precursor cell expressed, developmentally down-regulated gene 9 antibody, NEDD9 antibody, Nedd9 antibody

Gene ID: 4739