HRAS antibody

Catalog No. ABIN535258

Product Details anti-HRAS Antibody

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HRAS antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Mouse monoclonal antibody raised against synthetic peptide of HRAS.

Sequence: DIHQYREQIK RVKDSDDC

Specificity: This antibody reacts with human HRAS, an ubiquitously expressed 21 KDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals.

Cross-Reactivity: Human

Immunogen: A synthetic peptide corresponding to human HRAS.

Clone: H-RAS-03

Isotype: IgG1

Application Details

Application Notes: Western Blot (0.5-1 μg/mL)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS, pH 7.4 (0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.

References for anti-HRAS antibody (ABIN535258)

Pearl, Prodromou, Workman: "The Hsp90 molecular chaperone: an open and shut case for treatment." in: The Biochemical journal, Vol. 410, Issue 3, pp. 439-53, (2008) (PubMed).

Millson, Truman, Rácz, Hu, Panaretou, Nuttall, Mollapour, Söti, Piper et al.: "Expressed as the sole Hsp90 of yeast, the alpha and beta isoforms of human Hsp90 differ with regard to their capacities for activation of certain client proteins, whereas only Hsp90beta generates ..." in: The FEBS journal, Vol. 274, Issue 17, pp. 4453-63, (2007) (PubMed).

Hooven, Yamamoto, Jeffery: "Blind cavefish and heat shock protein chaperones: a novel role for hsp90alpha in lens apoptosis." in: The International journal of developmental biology, Vol. 48, Issue 8-9, pp. 731-8, (2004) (PubMed).

Target Details for HRAS

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Alternative Name: HRAS (HRAS Products)

Gene ID: 3265

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling