Mouse monoclonal antibody raised against native CD72.
Specificity
This antibody 3F3 reacts with CD72, a 39-43 KDa type II membrane glycoprotein (C-type lectin family). CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells, it is also present on follicular dendritic cells.
Cross-Reactivity
Human
Immunogen
Native purified CD72 from normal human lymphocytes from lymph node.
CD72
Reactivity: Human
FACS
Host: Mouse
Monoclonal
3F3
PE
Application Notes
Flow Cytometry (20 µL in human blood cells 100 µL in whole blood or 106 cells in a suspension) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.2 % BSA, 0.09 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing.
Mizrahi, Markel, Porgador, Bushkin, Mandelboim: "CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72." in: PLoS ONE, Vol. 2, Issue 9, pp. e818, (2007) (PubMed).
Kumanogoh, Shikina, Watanabe, Takegahara, Suzuki, Yamamoto, Takamatsu, Prasad, Mizui, Toyofuku, Tamura, Watanabe, Parnes, Kikutani: "Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment." in: International immunology, Vol. 17, Issue 10, pp. 1277-82, (2005) (PubMed).
Kumanogoh, Kikutani: "The CD100-CD72 interaction: a novel mechanism of immune regulation." in: Trends in immunology, Vol. 22, Issue 12, pp. 670-6, (2001) (PubMed).