FAS antibody (PE)

Catalog No. ABIN536045

Product Details anti-FAS Antibody (PE)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Purpose: Mouse monoclonal antibody raised against native FAS.

Specificity: This antibody reacts with CD95 (Fas/APO-1), a 46 KDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.
It seems that This antibody does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.

Cross-Reactivity: Human

Immunogen: Native purified FAS from human T cell lymphoma cell line HUT-78.

Clone: LT95

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (20 µL in human blood cells 100 µL in whole blood or 10⁶ cells in a suspension)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.2 % BSA, 0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.

References for anti-FAS antibody (PE) (ABIN536045)

Brumatti, Yon, Castro, Bueno-da-Silva, Jacysyn, Brunner, Amarante-Mendes: "Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide." in: Experimental cell research, Vol. 314, Issue 3, pp. 554-63, (2008) (PubMed).

Guo, Zhang, Tang, Cao: "Fas signal links innate and adaptive immunity by promoting dendritic-cell secretion of CC and CXC chemokines." in: Blood, Vol. 106, Issue 6, pp. 2033-41, (2005) (PubMed).

Drosopoulos, Roberts, Cermak, Sasazuki, Shirasawa, Andera, Pintzas: "Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 22856-67, (2005) (PubMed).

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95 / FAS (FAS Products)

Synonyms: ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody

Gene ID: 355

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity