Mouse monoclonal antibody raised against native CD58.
Specificity
This antibody reacts with CD58 (LFA-3), a 40-70 KDa glycoprotein distributed over many tissues, leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.
Cross-Reactivity
Human, Pig
Immunogen
Native purified CD58 from NALM-6 human pre-B cell line.
Flow Cytometry (20 µL in human blood cells 100 µL in whole blood or 106 cells in a suspension) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.2 % BSA, 0.09 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing.
Sheng, Li, Qi, Ji, Meng, Xie: "Investigation on correlation between expression of CD58 molecule and severity of hepatitis B." in: World journal of gastroenterology : WJG, Vol. 12, Issue 26, pp. 4237-40, (2006) (PubMed).
Ariel, Kukulansky, Raz, Hollander: "Distinct membrane localization and kinase association of the two isoforms of CD58." in: Cellular signalling, Vol. 16, Issue 6, pp. 667-73, (2004) (PubMed).
Veltroni, De Zen, Sanzari, Maglia, Dworzak, Ratei, Biondi, Basso, Gaipa: "Expression of CD58 in normal, regenerating and leukemic bone marrow B cells: implications for the detection of minimal residual disease in acute lymphocytic leukemia." in: Haematologica, Vol. 88, Issue 11, pp. 1245-52, (2003) (PubMed).