Western Blot (1:1000-1:5000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:400) Immunohistochemistry (Frozen sections) (1:200-1:400) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Buffer
In ascites
Storage
-20 °C
Storage Comment
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Song, Javanbakht, Perron, Park, Stremlau, Sodroski: "Retrovirus restriction by TRIM5alpha variants from Old World and New World primates." in: Journal of virology, Vol. 79, Issue 7, pp. 3930-7, (2005) (PubMed).
Stremlau, Owens, Perron, Kiessling, Autissier, Sodroski: "The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys." in: Nature, Vol. 427, Issue 6977, pp. 848-53, (2004) (PubMed).