ELISA (1:5000-1:24000) Western Blot (1:200-1:2000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Zhou, Lu, Park, Wilson-Chiru, Linton, Brady: "Tax interacts with P-TEFb in a novel manner to stimulate human T-lymphotropic virus type 1 transcription." in: Journal of virology, Vol. 80, Issue 10, pp. 4781-91, (2006) (PubMed).
Jang, Mochizuki, Zhou, Jeong, Brady, Ozato: "The bromodomain protein Brd4 is a positive regulatory component of P-TEFb and stimulates RNA polymerase II-dependent transcription." in: Molecular cell, Vol. 19, Issue 4, pp. 523-34, (2005) (PubMed).
Liu, Herrmann: "Differential localization and expression of the Cdk9 42k and 55k isoforms." in: Journal of cellular physiology, Vol. 203, Issue 1, pp. 251-60, (2005) (PubMed).