ECT2 antibody (pThr790)

Catalog No. ABIN538145

Product Details anti-ECT2 Antibody

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Binding Specificity: pThr790

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Monkey, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of ECT2.

Specificity: Reactivity occurs against human ECT2 protein at the pT790 residue. By ELISA This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human ECT2 is minimal by ELISA, but western blot shows specific staining of interphase cell lysates, which are predicted to contain non-phosphorylated ECT2.

Cross-Reactivity: Chicken, Chimpanzee, Dog, Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding T790 of human ECT2.

Application Details

Application Notes: ELISA (1:10000-1:40000)
Western Blot (1:1000-1:4000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-ECT2 antibody (ABIN538145)

Niiya, Xie, Lee, Inoue, Miki: "Inhibition of cyclin-dependent kinase 1 induces cytokinesis without chromosome segregation in an ECT2 and MgcRacGAP-dependent manner." in: The Journal of biological chemistry, Vol. 280, Issue 43, pp. 36502-9, (2005) (PubMed).

Zhao, Fang: "MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 37, pp. 13158-63, (2005) (PubMed).

Kim, Billadeau, Chen: "The tandem BRCT domains of Ect2 are required for both negative and positive regulation of Ect2 in cytokinesis." in: The Journal of biological chemistry, Vol. 280, Issue 7, pp. 5733-9, (2005) (PubMed).

Target Details for ECT2

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Alternative Name: ECT2 (ECT2 Products)

Synonyms: ECT2 antibody, arhgef31 antibody, xect2 antibody, AI528536 antibody, mKIAA4037 antibody, ARHGEF31 antibody, ect2 antibody, epithelial cell transforming 2 antibody, ect2 oncogene antibody, epithelial cell transforming 2 S homeolog antibody, ECT2 antibody, ect2 antibody, Ect2 antibody, ect2.S antibody

Gene ID: 1894

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization