Aurora Kinase B antibody (pThr232)

Catalog No. ABIN538167

Product Details anti-Aurora Kinase B Antibody

Target: Aurora Kinase B (AURKB)

Binding Specificity: pThr232

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of AURKB.

Specificity: Reactivity occurs against human Aurora Kinase B pT232 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A.

Cross-Reactivity: Chimpanzee, Cow, Dog, Human, Mouse, Pig, Rat

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding T232 of human AURKB.

Application Details

Application Notes: ELISA (1:10000-1:50000)
Western Blot (1:250-1:2000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-Aurora Kinase B antibody (ABIN538167)

Yasui, Urano, Kawajiri, Nagata, Tatsuka, Saya, Furukawa, Takahashi, Izawa, Inagaki: "Autophosphorylation of a newly identified site of Aurora-B is indispensable for cytokinesis." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12997-3003, (2004) (PubMed).

Kimura, Uchida, Takano, Kitagawa, Okano: "Cell cycle-dependent regulation of the human aurora B promoter." in: Biochemical and biophysical research communications, Vol. 316, Issue 3, pp. 930-6, (2004) (PubMed).

Gruneberg, Neef, Honda, Nigg, Barr: "Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2." in: The Journal of cell biology, Vol. 166, Issue 2, pp. 167-72, (2004) (PubMed).

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: Aurora kinase B (AURKB Products)

Synonyms: AIK2 antibody, AIM-1 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, IPL1 antibody, PPP1R48 antibody, STK12 antibody, STK5 antibody, aurkb-sv1 antibody, aurkb-sv2 antibody, AIRK2 antibody, AL022959 antibody, Aik2 antibody, Aim1 antibody, Ark2 antibody, STK-1 antibody, Stk12 antibody, Stk5 antibody, Aur B antibody, Aurora B antibody, Aurora-B antibody, CG6620 antibody, DmAIRK2 antibody, DmAurB antibody, DmAuroraB antibody, Dmel\\CG6620 antibody, IAL antibody, IAL1 antibody, Ial antibody, aurB antibody, aurora B antibody, ARK-2 antibody, XAIRK2 antibody, aik2 antibody, aim-1 antibody, aim1 antibody, airk2 antibody, ark2 antibody, ipl1 antibody, stk12 antibody, stk5 antibody, CHUNP6896 antibody, fa09g06 antibody, stka antibody, wu:fa09g06 antibody, aurkb antibody, AIRK2-B antibody, XAIRK2-B antibody, aurkb-a antibody, aurkb-b antibody, xAurora-B-B antibody, aurora kinase B antibody, aurora B antibody, aurora kinase B S homeolog antibody, AURKB antibody, Aurkb antibody, aurB antibody, aurkb antibody, aurkb.S antibody

Gene ID: 9212

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades