PERK antibody (AA 601-1115)

Catalog No. ABIN538307

Product Details anti-PERK Antibody

Target: PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

Binding Specificity: AA 601-1115

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PERK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Purpose: Rabbit polyclonal antibody raised against partial recombinant Eif2ak3.

Cross-Reactivity: Mouse

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: Recombinant protein corresponding to amino acids 601-1115 of mouse Eif2ak3.

Application Details

Application Notes: Western Blot (1:500-1:3000)
Immunoprecipitation (10-30 µL)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-PERK antibody (ABIN538307)

Lee, Surh: "Nrf2 as a novel molecular target for chemoprevention." in: Cancer letters, Vol. 224, Issue 2, pp. 171-84, (2005) (PubMed).

Cullinan, Zhang, Hannink, Arvisais, Kaufman, Diehl: "Nrf2 is a direct PERK substrate and effector of PERK-dependent cell survival." in: Molecular and cellular biology, Vol. 23, Issue 20, pp. 7198-209, (2003) (PubMed).

Ma, Vattem, Wek: "Dimerization and release of molecular chaperone inhibition facilitate activation of eukaryotic initiation factor-2 kinase in response to endoplasmic reticulum stress." in: The Journal of biological chemistry, Vol. 277, Issue 21, pp. 18728-35, (2002) (PubMed).

Target Details for PERK

Target: PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

Alternative Name: EIF2AK3 (EIF2AK3 Products)

Synonyms: EIF2AK3 antibody, AI427929 antibody, Pek antibody, Perk antibody, PEK antibody, PERK antibody, WRS antibody, eukaryotic translation initiation factor 2 alpha kinase 3 antibody, EIF2AK3 antibody, Eif2ak3 antibody

Gene ID: 13666

Pathways: Hormone Transport, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response