Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Lee, Perrem, Harper, Lu, Zhou: "The F-box protein FBX4 targets PIN2/TRF1 for ubiquitin-mediated degradation and regulates telomere maintenance." in: The Journal of biological chemistry, Vol. 281, Issue 2, pp. 759-68, (2006) (PubMed).
Lin, Barbash, Kumar, Weber, Harper, Klein-Szanto, Rustgi, Fuchs, Diehl: "Phosphorylation-dependent ubiquitination of cyclin D1 by the SCF(FBX4-alphaB crystallin) complex." in: Molecular cell, Vol. 24, Issue 3, pp. 355-66, (2006) (PubMed).
den Engelsman, Keijsers, de Jong, Boelens: "The small heat-shock protein alpha B-crystallin promotes FBX4-dependent ubiquitination." in: The Journal of biological chemistry, Vol. 278, Issue 7, pp. 4699-704, (2003) (PubMed).