PRKDC antibody (pThr2609)

Catalog No. ABIN538467

Product Details anti-PRKDC Antibody

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Binding Specificity: pThr2609

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKDC antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKDC.

Specificity: This phospho-specific polyclonal antibody reacts with phosphorylated pT2609 of human DNA PKcs. Reactivity with non-phosphorylated human DNA PKcs is minimal.

Cross-Reactivity: Chicken, Chimpanzee, Dog, Horse, Human, Mouse, Rat

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding T2609 of human PRKDC.

Application Details

Application Notes: ELISA (1:300000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-PRKDC antibody (ABIN538467)

Panta, Kaur, Cavin, Cortés, Mercurio, Lothstein, Sweatman, Israel, Arsura et al.: "ATM and the catalytic subunit of DNA-dependent protein kinase activate NF-kappaB through a common MEK/extracellular signal-regulated kinase/p90(rsk) signaling pathway in response to distinct forms of ..." in: Molecular and cellular biology, Vol. 24, Issue 5, pp. 1823-35, (2004) (PubMed).

Sawchuk, Mansilla-Soto, Alarcon, Singha, Langen, Bianchi, Lees-Miller, Nussenzweig, Cortes: "Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 29821-31, (2004) (PubMed).

Graham, Gustin, Rivera, Kuyumcu-Martinez, Choe, Lloyd, Sarnow, Utz: "Proteolytic cleavage of the catalytic subunit of DNA-dependent protein kinase during poliovirus infection." in: Journal of virology, Vol. 78, Issue 12, pp. 6313-21, (2004) (PubMed).

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Alternative Name: DNA-PKcs / PRKDC / XRCC7 (PRKDC Products)

Synonyms: DNA-PKcs antibody, DNAPK antibody, DNPK1 antibody, HYRC antibody, HYRC1 antibody, XRCC7 antibody, p350 antibody, DNA-PKCS antibody, dna-pkcs antibody, dnapk antibody, dnpk1 antibody, hyrc antibody, hyrc1 antibody, prkdc-A antibody, xrcc7 antibody, PRKDC antibody, AI326420 antibody, AU019811 antibody, DNAPDcs antibody, DOXNPH antibody, dxnph antibody, p460 antibody, scid antibody, slip antibody, protein kinase, DNA-activated, catalytic polypeptide antibody, protein kinase, DNA-activated, catalytic polypeptide L homeolog antibody, DNA-dependent protein kinase catalytic subunit antibody, protein kinase, DNA activated, catalytic polypeptide antibody, PRKDC antibody, prkdc.L antibody, LOC100633457 antibody, Prkdc antibody

Gene ID: 5591

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response