MDM2 antibody (pSer185)

Catalog No. ABIN538477

Product Details anti-MDM2 Antibody

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Binding Specificity: pSer185

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of Mdm2.

Specificity: Reactivity occurs against Mouse MDM2 pS185 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated mouse MDM2 is minimal by ELISA and western blot.

Cross-Reactivity: Mouse

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S185 of mouse Mdm2.

Application Details

Application Notes: ELISA (1:3000-1:12000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-MDM2 antibody (ABIN538477)

Stros, Muselíková-Polanská, Pospísilová, Strauss: "High-affinity binding of tumor-suppressor protein p53 and HMGB1 to hemicatenated DNA loops." in: Biochemistry, Vol. 43, Issue 22, pp. 7215-25, (2004) (PubMed).

Aslanian, Iaquinta, Verona, Lees: "Repression of the Arf tumor suppressor by E2F3 is required for normal cell cycle kinetics." in: Genes & development, Vol. 18, Issue 12, pp. 1413-22, (2004) (PubMed).

Pospísilová, Siligan, Ban, Jug, Kovar: "Constitutive and DNA damage inducible activation of pig3 and MDM2 genes by tumor-derived p53 mutant C277Y." in: Molecular cancer research : MCR, Vol. 2, Issue 5, pp. 296-304, (2004) (PubMed).

Target Details for MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Alternative Name: MDM2 (MDM2 Products)

Synonyms: ACTFS antibody, HDMX antibody, hdm2 antibody, zgc:109834 antibody, 1700007J15Rik antibody, AA415488 antibody, Mdm-2 antibody, MDM2 proto-oncogene antibody, MDM2 proto-oncogene S homeolog antibody, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) antibody, MDM2 oncogene, E3 ubiquitin protein ligase antibody, transformed mouse 3T3 cell double minute 2 antibody, MDM2 antibody, mdm2.S antibody, Mdm2 antibody, mdm2 antibody

Gene ID: 17246

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway