FOXO1 antibody (pSer256)

Catalog No. ABIN539166

Product Details anti-FOXO1 Antibody

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Binding Specificity: pSer256

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of FOXO1.

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S256 of human FOXO1.

Application Details

Application Notes: Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS (without Mg²⁺ and Ca²⁺), 150 mM NaCl, pH 7.4 (50 % glycerol, 0.02 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-FOXO1 antibody (ABIN539166)

Sajan, Ivey, Farese: "BMI-related progression of atypical PKC-dependent aberrations in insulin signaling through IRS-1, Akt, FoxO1 and PGC-1α in livers of obese and type 2 diabetic humans." in: Metabolism: clinical and experimental, Vol. 64, Issue 11, pp. 1454-65, (2015) (PubMed).

Sajan, Ivey, Lee, Mastorides, Jurczak, Samuels, Shulman, Braun, Leitges, Farese: "PKC? haploinsufficiency prevents diabetes by a mechanism involving alterations in hepatic enzymes." in: Molecular endocrinology (Baltimore, Md.), Vol. 28, Issue 7, pp. 1097-107, (2014) (PubMed).

Sajan, Ivey, Lee, Farese: "Hepatic insulin resistance in ob/ob mice involves increases in ceramide, aPKC activity, and selective impairment of Akt-dependent FoxO1 phosphorylation." in: Journal of lipid research, Vol. 56, Issue 1, pp. 70-80, (2014) (PubMed).

Target Details for FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Alternative Name: FOXO1A (FOXO1 Products)

Synonyms: FKH1 antibody, FKHR antibody, FOXO1A antibody, FoxO1a.1 antibody, foxo1 antibody, zgc:153388 antibody, Fkhr antibody, Foxo1a antibody, AI876417 antibody, Afxh antibody, Fkhr1 antibody, fkh1 antibody, fkhr antibody, foxo1a antibody, xFoxO1 antibody, FoxO1A antibody, foxo1a.2 antibody, forkhead box O1 antibody, forkhead box O1 a antibody, forkhead box O1 L homeolog antibody, forkhead box O1 b antibody, FOXO1 antibody, foxo1a antibody, Foxo1 antibody, foxo1 antibody, foxo1.L antibody, foxo1b antibody

Gene ID: 2308

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling