Western Blotting (WB), ELISA, Immunoprecipitation (IP)
Purpose
Rabbit polyclonal antibody raised against synthetic peptide of MYL9.
Specificity
This affinity purified antibody is directed against the regulatory light chain of smooth and non- muscle myosin. This antibody detects both unphosphorylated and monophosphorylated forms of the protein.
Cross-Reactivity
Human, Mouse, Rat
Characteristics
Antibody Reactive Against Synthetic Peptide.
Immunogen
A synthetic peptide corresponding to amino acids 12-27 of human MYL9.
MYL9
Reactivity: Human
WB, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA (1:5000-1:20000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Iwasaki, Murata-Hori, Ishitobi, Hosoya: "Diphosphorylated MRLC is required for organization of stress fibers in interphase cells and the contractile ring in dividing cells." in: Cell structure and function, Vol. 26, Issue 6, pp. 677-83, (2002) (PubMed).