Rabbit polyclonal antibody raised against synthetic peptide of SMAD2.
Specificity
This antibody is Smad2 specific, and reactivity to other Smad proteins (specifically Smad1, Smad3, Smad4, and Smad7) is not detected in over-expressed cell lysates.
Cross-Reactivity
Human, Mouse, Rat
Characteristics
Antibody Reactive Against Synthetic Peptide.
Immunogen
A synthetic peptide corresponding to internal region of human SMAD2.
ELISA (1:100000) Western Blot (1:1000-1:3000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Bruna, Darken, Rojo, Ocaña, Peñuelas, Arias, Paris, Tortosa, Mora, Baselga, Seoane: "High TGFbeta-Smad activity confers poor prognosis in glioma patients and promotes cell proliferation depending on the methylation of the PDGF-B gene." in: Cancer cell, Vol. 11, Issue 2, pp. 147-60, (2007) (PubMed).
Liapis, Mylona, Alexandrou, Giannopoulou, Nikolaou, Markaki, Keramopoulos, Nakopoulou: "Effect of the different phosphorylated Smad2 protein localizations on the invasive breast carcinoma phenotype." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 115, Issue 2, pp. 104-14, (2007) (PubMed).
Yang, Huang, Chen, Lin, Chen: "Leptin augments myofibroblastic conversion and fibrogenic activity of human peritoneal mesothelial cells: a functional implication for peritoneal fibrosis." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 22, Issue 3, pp. 756-62, (2007) (PubMed).
Target
SMAD2
(SMAD, Mothers Against DPP Homolog 2 (SMAD2))