Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purpose
Rabbit polyclonal antibody raised against full length recombinant S100A1/S100B.
Specificity
A 98 % homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94 % homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Homologies for the S100 beta chain are similar.
Cross-Reactivity
Cow, Dog, Mouse, Rat
Characteristics
Antibody Reactive Against Recombinant Protein.
Immunogen
Recombinant protein corresponding to full length bovine S100A1/S100B.
S100A/B
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
1-B-657
HRP
Application Notes
ELISA (1:5000-1:25000) Western Blot (1:500-1:3000) Immunohistochemistry (1:200-1:2000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot after reconstitution to avoid repeated freezing and thawing.
Charron, Doudnikoff, Canron, Li, Véga, Marais, Baufreton, Vital, Oliet, Bezard: "Astrocytosis in parkinsonism: considering tripartite striatal synapses in physiopathology?" in: Frontiers in aging neuroscience, Vol. 6, pp. 258, (2014) (PubMed).
Charron, Doudnikoff, Laux, Berthet, Porras, Canron, Barroso-Chinea, Li, Qin, Nosten-Bertrand, Giros, Delalande, Van Dorsselaer, Vital, Goumon, Bezard: "Endogenous morphine-like compound immunoreactivity increases in parkinsonism." in: Brain : a journal of neurology, Vol. 134, Issue Pt 8, pp. 2321-38, (2011) (PubMed).