LC3B antibody (AA 1-50)

Catalog No. ABIN540836

Product Details anti-LC3B Antibody

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Binding Specificity: AA 1-50

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LC3B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of MAP1LC3B.

Specificity: This antibody is specific to the B isoform of LC3.

Cross-Reactivity: Human, Mouse

Immunogen: A synthetic peptide corresponding to amino acids 1-50 at N-terminus of human MAP1LC3B.

Application Details

Application Notes: Flow Cytometry (1:200)
Immunocytochemistry (1:2000)
Immunofluorescence (1:2000)
Immunohistochemistry (Frozen sections) (1:2000)
Western Blot (1:3000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.02 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-LC3B antibody (ABIN540836)

Rodriguez-Ortiz, Flores, Valenzuela, Rodriguez, Zumkehr, Tran, Kimonis, Kitazawa: "The Myoblast C2C12 Transfected with Mutant Valosin-Containing Protein Exhibits Delayed Stress Granule Resolution on Oxidative Stress." in: The American journal of pathology, Vol. 186, Issue 6, pp. 1623-34, (2016) (PubMed).

Pyo, Nah, Kim, Lee, Heo, Lee, Jung: "Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death." in: Autophagy, Vol. 4, Issue 3, pp. 315-21, (2008) (PubMed).

Tafani, Schito, Anwar, Indelicato, Sale, Di Vito, Morgante, Beraldi, Makovec, Letari, Caselli, Spadafora, Pucci, Russo: "Induction of autophagic cell death by a novel molecule is increased by hypoxia." in: Autophagy, Vol. 4, Issue 8, pp. 1042-53, (2008) (PubMed).

Gao, Yeh, Lu, Hsu, Chen, Lee, Feng, Chen, Kuo, Cheng: "OSU-03012, a novel celecoxib derivative, induces reactive oxygen species-related autophagy in hepatocellular carcinoma." in: Cancer research, Vol. 68, Issue 22, pp. 9348-57, (2008) (PubMed).

Aoki, Takada, Kondo, Sawaya, Aggarwal, Kondo: "Evidence that curcumin suppresses the growth of malignant gliomas in vitro and in vivo through induction of autophagy: role of Akt and extracellular signal-regulated kinase signaling pathways." in: Molecular pharmacology, Vol. 72, Issue 1, pp. 29-39, (2007) (PubMed).

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: LC3B (MAP1LC3B Products)

Synonyms: ATG8F antibody, LC3B antibody, MAP1A/1BLC3 antibody, MAP1LC3B-a antibody, Map1lc3 antibody, Mpl3 antibody, zbs559 antibody, 1010001C15Rik antibody, Atg8 antibody, LC3b antibody, zgc:56434 antibody, wu:fb60g11 antibody, map1lc3b antibody, MGC76283 antibody, MAP1LC3B antibody, microtubule associated protein 1 light chain 3 beta antibody, microtubule-associated protein 1 light chain 3 beta antibody, microtubule associated protein 1 light chain 3 beta L homeolog antibody, MAP1LC3B antibody, Map1lc3b antibody, map1lc3b antibody, map1lc3b.L antibody

Gene ID: 81631

Pathways: Autophagy