Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Purpose
Rabbit polyclonal antibody raised against full length recombinant MARCKS.
Specificity
The MARCKS protein runs at about 80KDa on SDS-PAGE gels, although the real molecular weight is much lower, at about 31.5KDa. The reason for this is probably because the protein is extremely rich in acidic residues. Such proteins appear to bind less SDS than the average protein, and so migrate in PAGE more slowly than the normal protein.
Immunogen
Recombinant protein corresponding to full length MARCKS.
Immunocytochemistry (1:5000) Immunofluorescence (1:500-1:1000) Western Blot (1:20000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In antiserum
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Target
MARCKS
(Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))