IRS1 antibody (pTyr612)

Catalog No. ABIN540934

Product Details anti-IRS1 Antibody

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Binding Specificity: pTyr612

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of IRS1.

Sequence: DGYMP

Specificity: This antibody recognizes ~165 KDa of human IRS1 (pTyr 612).

Cross-Reactivity: Human, Mouse, Rat

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Y612 of human IRS1.

Isotype: IgG

Application Details

Application Notes: Western Blot (0.1-1 μg/mL)
ELISA (0.01-0.1 μg/mL)
Immunoprecipitation (2-5 μg/mL)
Immunohistochemistry (0.5-2 μg/mL)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In TBS, pH 7.2 (BSA, 10 % Proclin300)

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-IRS1 antibody (ABIN540934)

Li, Soos, Li, Wu, Degennaro, Sun, Littman, Birnbaum, Polakiewicz: "Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101)." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 45304-7, (2004) (PubMed).

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS1 (IRS1 Products)

Synonyms: HIRS-1 antibody, G972R antibody, IRS-1 antibody, IRS1IRM antibody, irs1 antibody, irsu antibody, IRS1 antibody, irs-1 antibody, irs1-a antibody, insulin receptor substrate 1 antibody, insulin receptor substrate 2 antibody, insulin receptor substrate 1 S homeolog antibody, IRS1 antibody, Irs1 antibody, irs2 antibody, irs1.S antibody

Gene ID: 3667

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process