Western Blot (0.5-1 μg/mL) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.02 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for three months. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Xu, Parmar, Roux, Balbis, Dumas, Chevalier, Posner: "Epidermal growth factor-induced vacuolar (H+)-atpase assembly: a role in signaling via mTORC1 activation." in: The Journal of biological chemistry, Vol. 287, Issue 31, pp. 26409-22, (2012) (PubMed).
Sofer, Lei, Johannessen, Ellisen: "Regulation of mTOR and cell growth in response to energy stress by REDD1." in: Molecular and cellular biology, Vol. 25, Issue 14, pp. 5834-45, (2005) (PubMed).
Ellisen, Ramsayer, Johannessen, Yang, Beppu, Minda, Oliner, McKeon, Haber: "REDD1, a developmentally regulated transcriptional target of p63 and p53, links p63 to regulation of reactive oxygen species." in: Molecular cell, Vol. 10, Issue 5, pp. 995-1005, (2002) (PubMed).
Shoshani, Faerman, Mett, Zelin, Tenne, Gorodin, Moshel, Elbaz, Budanov, Chajut, Kalinski, Kamer, Rozen, Mor, Keshet, Leshkowitz, Einat, Skaliter, Feinstein: "Identification of a novel hypoxia-inducible factor 1-responsive gene, RTP801, involved in apoptosis." in: Molecular and cellular biology, Vol. 22, Issue 7, pp. 2283-93, (2002) (PubMed).