Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purpose
Goat polyclonal antibody raised against synthetic peptide of CAMK2A.
Sequence
C-PRTAQSEETR VWHR
Specificity
Approx 55 and 65 KDa bands observed in human brain (cerebellum) and mouse brain lysates (calculated MW of 55.3 KDa according to human NP_057065.2 and of 54.1 KDa according to human NP_741960.1 and mouse NP_803126.1).
Cross-Reactivity
Dog, Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to internal region of human CAMK2A.
CAMK2A
Reactivity: Human
WB, ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA (1:64000) Western Blot (0.1-0.3 μg/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 μg/mL) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.5 mg/mL
Buffer
In Tris saline, pH 7.3 (0.5 % BSA, 0.02 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Lee, Lin, Luo, Lee, Lee, Aird, Hwang, Yang et al.: "Tumor necrosis factor-alpha enhances neutrophil adhesiveness: induction of vascular cell adhesion molecule-1 via activation of Akt and CaM kinase II and modifications of histone acetyltransferase and ..." in: Molecular pharmacology, Vol. 73, Issue 5, pp. 1454-64, (2008) (PubMed).
Target
CAMK2A
(Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))