Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))
Purpose
Rabbit polyclonal antibody raised against partial NTRK1.
Specificity
Specificity was demonstrated by immunohistochemistry. When used for immunohistochemistry in rat dorsal root ganglia, staining is restricted to the known distribution of TrkA, that is in small, nociceptive neurons.
NTRK1
Reactivity: Human, Mouse, Rat
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA (1:5000) Immunohistochemistry (1:1000) Western Blotting (1:1000-1:2500) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
Lyophilized from PBS
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for short term. Store at -20°C on dry atmosphere for long term. After reconstitution with 100 uL of sterile water, store at -20°C. Aliquot to avoid repeated freezing and thawing.
Gearhart, Middlemore, Terry: "ELISA methods to measure cholinergic markers and nerve growth factor receptors in cortex, hippocampus, prefrontal cortex, and basal forebrain from rat brain." in: Journal of neuroscience methods, Vol. 150, Issue 2, pp. 159-73, (2006) (PubMed).
Zhang, Lai, Tse, Shum, Chan: "Expression of Trk receptors in otolith-related neurons in the vestibular nucleus of rats." in: Brain research, Vol. 1062, Issue 1-2, pp. 92-100, (2005) (PubMed).
Target
TRKA (NTRK1)
(Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))