SMURF2 antibody (SMAD Specific E3 Ubiquitin Protein Ligase 2)

Details for Product anti-SMURF2 Antibody No. ABIN542769
Antigen
Reactivity
Human
89
39
21
Host
Rabbit
82
6
1
Clonality
Polyclonal
Conjugate
This SMURF2 antibody is un-conjugated
8
6
6
4
4
4
3
3
3
3
3
3
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
61
41
39
20
13
13
8
2
2
1
1
Options
Purpose Rabbit polyclonal antibody raised against synthetic peptide of SMURF2.
Immunogen A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human SMURF2.
Antigen
Alternative Name SMURF2 (SMURF2 Antibody Abstract)
Application Notes Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
Restrictions For Research Use only
Buffer In PBS (0.09 % sodium azide)
Storage 4 °C,-20 °C
Storage Comment Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Supplier Images
Image no. 1 for anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) antibody (ABIN542769) The SMURF2 polyclonal antibody is used in Western blot to detect SMURF2 in mouse bra...
Image no. 2 for anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) antibody (ABIN542769) Formalin-fixed and paraffin-embedded human cancer tissue reacted with SMURF2 polyclon...
Product cited in: Zhang, Chang, Gehling, Hemmati-Brivanlou, Derynck: "Regulation of Smad degradation and activity by Smurf2, an E3 ubiquitin ligase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 3, pp. 974-9, 2001 (PubMed).

Kavsak, Rasmussen, Causing, Bonni, Zhu, Thomsen, Wrana: "Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF beta receptor for degradation." in: Molecular cell, Vol. 6, Issue 6, pp. 1365-75, 2001 (PubMed).

Lin, Liang, Feng: "Smurf2 is a ubiquitin E3 ligase mediating proteasome-dependent degradation of Smad2 in transforming growth factor-beta signaling." in: The Journal of biological chemistry, Vol. 275, Issue 47, pp. 36818-22, 2001 (PubMed).