Western Blot (1:1000) Immunohistochemistry (1:10-50) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.09 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Molnár, Migh, Szikora, Kalmár, Végh, Deák, Barkó, Bugyi, Orfanos, Kovács, Juhász, Váró, Nyitrai, Sparrow, Mihály: "DAAM is required for thin filament formation and Sarcomerogenesis during muscle development in Drosophila." in: PLoS genetics, Vol. 10, Issue 2, pp. e1004166, (2014) (PubMed).
Liu, Sato, Khadka, Bharti, Diaz, Runnels, Habas: "Mechanism of activation of the Formin protein Daam1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 1, pp. 210-5, (2008) (PubMed).
Lu, Meng, Poy, Maiti, Goode, Eck: "Structure of the FH2 domain of Daam1: implications for formin regulation of actin assembly." in: Journal of molecular biology, Vol. 369, Issue 5, pp. 1258-69, (2007) (PubMed).
Yamashita, Higashi, Suetsugu, Sato, Ikeda, Shirakawa, Kita, Takenawa, Horiuchi, Fukai, Nureki: "Crystal structure of human DAAM1 formin homology 2 domain." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 12, Issue 11, pp. 1255-65, (2007) (PubMed).
Target
DAAM1
(Dishevelled Associated Activator of Morphogenesis 1 (DAAM1))