Western Blot (1:1000) Immunohistochemistry (1:10-50) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.09 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Yamaguchi, Wallace, Magenheimer, Hempson, Grantham, Calvet: "Calcium restriction allows cAMP activation of the B-Raf/ERK pathway, switching cells to a cAMP-dependent growth-stimulated phenotype." in: The Journal of biological chemistry, Vol. 279, Issue 39, pp. 40419-30, (2004) (PubMed).
Frattini, Ferrario, Bressan, Balestra, De Cecco, Mondellini, Bongarzone, Collini, Gariboldi, Pilotti, Pierotti, Greco: "Alternative mutations of BRAF, RET and NTRK1 are associated with similar but distinct gene expression patterns in papillary thyroid cancer." in: Oncogene, Vol. 23, Issue 44, pp. 7436-40, (2004) (PubMed).
Loewe, Kittler, Fischer, Faé, Wolff, Petzelbauer: "BRAF kinase gene V599E mutation in growing melanocytic lesions." in: The Journal of investigative dermatology, Vol. 123, Issue 4, pp. 733-6, (2004) (PubMed).