NLK
Reactivity: Human
WB, IF
Host: Mouse
Polyclonal
unconjugated
Application Notes
Western Blot (1:1000) Immunohistochemistry (1:50-100) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.09 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Yasuda, Tsuchiya, Yamada, Sakamoto, Sekiya, Hirohashi: "Nemo-like kinase induces apoptosis in DLD-1 human colon cancer cells." in: Biochemical and biophysical research communications, Vol. 308, Issue 2, pp. 227-33, (2003) (PubMed).
Ishitani, Ninomiya-Tsuji, Matsumoto: "Regulation of lymphoid enhancer factor 1/T-cell factor by mitogen-activated protein kinase-related Nemo-like kinase-dependent phosphorylation in Wnt/beta-catenin signaling." in: Molecular and cellular biology, Vol. 23, Issue 4, pp. 1379-89, (2003) (PubMed).
Ishitani, Kishida, Hyodo-Miura, Ueno, Yasuda, Waterman, Shibuya, Moon, Ninomiya-Tsuji, Matsumoto: "The TAK1-NLK mitogen-activated protein kinase cascade functions in the Wnt-5a/Ca(2+) pathway to antagonize Wnt/beta-catenin signaling." in: Molecular and cellular biology, Vol. 23, Issue 1, pp. 131-9, (2002) (PubMed).