GCK antibody (C-Term)

Catalog No. ABIN544490

Product Details anti-GCK Antibody

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of GCK.

Cross-Reactivity: Human, Mouse

Immunogen: A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human GCK.

Application Details

Application Notes: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-GCK antibody (ABIN544490)

Gloyn, Noordam, Willemsen, Ellard, Lam, Campbell, Midgley, Shiota, Buettger, Magnuson, Matschinsky, Hattersley: "Insights into the biochemical and genetic basis of glucokinase activation from naturally occurring hypoglycemia mutations." in: Diabetes, Vol. 52, Issue 9, pp. 2433-40, (2003) (PubMed).

Pruhova, Ek, Lebl, Sumnik, Saudek, Andel, Pedersen, Hansen: "Genetic epidemiology of MODY in the Czech republic: new mutations in the MODY genes HNF-4alpha, GCK and HNF-1alpha." in: Diabetologia, Vol. 46, Issue 2, pp. 291-5, (2003) (PubMed).

Rizzo, Magnuson, Drain, Piston: "A functional link between glucokinase binding to insulin granules and conformational alterations in response to glucose and insulin." in: The Journal of biological chemistry, Vol. 277, Issue 37, pp. 34168-75, (2002) (PubMed).

Target Details for GCK

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Alternative Name: Glucokinase / Hexokinase-4 (GCK Products)

Synonyms: FGQTL3 antibody, GK antibody, GLK antibody, HHF3 antibody, HK4 antibody, HKIV antibody, HXKP antibody, LGLK antibody, MODY2 antibody, Gk antibody, Gls006 antibody, GCK antibody, BA4487 antibody, glk antibody, hk4 antibody, hhf3 antibody, hkiv antibody, hxkp antibody, gck-A antibody, mody2 antibody, glucokinase antibody, GLUKA antibody, RNGK2 antibody, glucokinase antibody, glucokinase (hexokinase 4) L homeolog antibody, glucokinase (hexokinase 4) antibody, GCK antibody, Gck antibody, glcK antibody, Bphyt_1025 antibody, Halhy_6548 antibody, gk antibody, gck.L antibody, gck antibody

Gene ID: 2645

Pathways: MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process